Which method is commonly used for cell lysis?

Prepare for the UofT BCH210H1 Biochemistry I midterm with exam-like questions. Access detailed solutions and explanations for proteins, lipids, and metabolism topics. Strengthen your understanding and excel on test day!

The correct response highlights mechanical grinding and osmotic pressure as common methods for cell lysis. Mechanical grinding involves physically disrupting the cellular structure, typically using a mortar and pestle or homogenizer, allowing the components within the cell to be released into a solution. This method is effective for breaking open cells, particularly those that are tough or resilient.

Osmotic pressure, on the other hand, utilizes differences in solute concentrations between the inside of the cell and the external environment. When placed in a hypotonic solution, water enters the cell, causing it to swell and eventually burst, a process known as osmotic lysis. This method is especially useful for cells that are delicate and may break apart easily when exposed to mechanical forces.

The combination of these techniques makes this approach versatile for various cell types, particularly in laboratory settings where extracting cellular components is necessary for further analysis.

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