What does the Lineweaver-Burk equation illustrate?

The Lineweaver-Burk equation is a double-reciprocal plot of the Michaelis-Menten equation, primarily used to determine important kinetic parameters of enzyme-catalyzed reactions. This equation represents the relationship between the reciprocal of the reaction velocity (1/V) and the reciprocal of the substrate concentration (1/[S]).

This linear transformation allows for easier determination of the maximum velocity (Vmax) and the Michaelis constant (Km). By plotting the reciprocal values, a straight line can be formed where the y-intercept equals 1/Vmax and the x-intercept equals -1/Km. Thus, the correct choice highlights how the Lineweaver-Burk equation effectively illustrates the reciprocal values of the final reaction rate and the substrate concentration, facilitating the analysis of enzyme kinetics.