What does a change in concentration during binding interactions indicate?

A change in concentration during binding interactions indicates overall reaction progress because it reflects the dynamics of both reactants and products in a biochemical reaction. In a typical enzyme-substrate interaction, as substrate binds to the enzyme, it is converted into product, leading to a measurable change in the concentration of both the substrate and the product over time. This change allows researchers to assess the efficiency and kinetics of the enzymatic reaction. Monitoring these concentrations provides insight into the reaction's progression, illustrating not just substrate consumption, but also product formation and, importantly, the balance between these two processes throughout the reaction cycle. Therefore, it is this comprehensive view of concentration changes that signifies overall reaction progress, rather than focusing solely on one aspect such as degradation or specific consumption or product formation.